Part:BBa_K1413022:Design

J23114-B0034-bphR2 mutated-B0015

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 522
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 306
  

Design Notes

bphR2 gene has been mutated because of its pstI site in its sequence.

Source

J23114, rbs and terminator come from the registry. bphr2 has been synthetized (it's the part BBa_K1413021)